人组织激肽释放酶（Kallikrein8）蛋白在宫颈癌中的表达及临床意义

【摘要】目的   通过研究Kallikrein8(klk8)蛋白在宫颈癌的表达，探讨klk8蛋白在宫颈癌发病中的作用。方法   选择正常宫颈组11例，慢性宫颈炎组13例，宫颈癌组43例，应用免疫组化方法，研究KLK8基因的表达产物即KLK8蛋白在宫颈癌组织内表达。结果   KLK8蛋白定位于宫颈上皮细胞胞浆内。宫颈浸润癌组织，慢性宫颈炎组织及正常宫颈组织中KLK8蛋白的阳性表达率分别为69.8%，15.4%，0，其中宫颈癌与正常宫颈组织间KLK8蛋白阳性表达率有明显差异（P＜0.01），与慢性宫颈炎相比KLK8蛋白阳性表达率也有明显差异（P＜0.01），而正常宫颈和慢性宫颈炎组织间KLK8蛋白阳性表达率无明显差异（P＞0.05）。结论  ① KLK8蛋白在宫颈癌中的表达明显高于正常和慢性宫颈炎的人。②KLK8蛋白在肿瘤的入侵和转移中发挥着重要的作用，参与了宫颈癌的形成机制，并且将成为宫颈癌诊断的有力工具和治疗的新靶点。

【关键词】宫颈癌；KLK8蛋白  免疫组织化学

The Expression of Kallikrein8 Protein is Associate with the Cervical Carcinoma

【Abstract】Objective   To examine the expression of the cervical carcinoma,and to assess the effects of KLK8 protein in cervical carcinoma. Methods   The expression of KLK8 protein was examined by Immunohistochemisty in 67 cases that composed of 11 cases with normal cervical tissues,13 cases with chronic cervicitis tissues and 43 cases with cervical carcinoma tissues .Results   KLK8 protein was located in cervical epithelial plasma.The positive expression rate of KLK8 protein was 69.8% in cervical carcinoma tissues；15.4% in  chronic cervicitis tissues and 0% in normal cervical tissues,The expression rate of  KLK8 protein was significantly higher in cells of cervical cancer(69.8%) than those in normal cervical tissues(0%)(p＜0.01）,and than those in chronic cervicitis tissues (15.4%)(p＜0.01 .but there was no significant difference between normal cervical tissues and  chronic cervicitis (p﹥0.05).Conclusion   ①The positive expression rate of KLK8 protein in cells of cervical cancer  was higher than those in normal and chronic cervicitis human. ②KLK8 protein play an important role in invasion and metastasis ,and participute in the pathogenesis of cervical cancer. It may prove to be a useful diagnostic tool or a novel molecular target for cervical cancer.

【Key Words】  Cervical carcinoma；KLK8 protein；Immunohistochemistry

宫颈癌（cervical cancer）是最常见的妇科恶性肿瘤之一。其发病率在妇女恶性肿瘤中居第二，仅次于乳腺癌，而在发展中国家则高居首位，严重威胁着妇女的健康及生命。早期宫颈癌5年总生存率可达71.6%，但宫颈浸润癌的疗效及总体生存率仍旧不高。除了传统的临床病理学指标外，能找到与肿瘤生物学行为关系密切的，客观的，可重复性好的生物学因素，做到宫颈癌早期诊断和治疗前后及预后的全面评估，是医生们刻不容辞的责任。人组织激肽释放酶（Kallikrein,KLK）是近几年新兴的肿瘤相关生物学标志，是一组大的丝氨酸蛋白酶家族，在人体内受激素调控和妇科肿瘤关系密切。本研究采用Kallikrein8多克隆抗体，应用免疫组化三步法，研究KLK8基因的表达产物即KLK8蛋白在宫颈癌组织内定位、分布及KLK8蛋白表达与宫颈癌临床病理关系，探讨其在宫颈癌发生、发展、入侵和转移中发挥重要作用。为宫颈癌的诊断、监测和治疗提供重要理论依据。